【摘要】 目的:探讨结肠腺瘤性息肉蛋白(APC)、β-连接素(β-catenin)和E-钙黏素(E-cadherin)在大肠癌发生、发展中的作用。方法:采用免疫组化方法检测30例正常大肠黏膜、30例大肠腺瘤、10例大肠腺瘤恶变及50例大肠癌组织中APC、β-catenin和E-cadherin蛋白的表达情况。结果:大肠癌和大肠腺瘤恶变APC阳性率分别为44.0%,40.0%,显著低于大肠腺瘤(86.7%)和正常大肠黏膜(100%)。大肠癌、大肠腺瘤恶变和大肠腺瘤β-catenin胞浆(胞核)异位表达率分别为62.0%,50.0%,30.0%,显著高于正常大肠黏膜(0%),大肠癌β-catenin异位表达率显著高于大肠腺瘤。大肠癌中β-catenin、E-cadherin膜表达缺失率为46.0%,34.0%,显著高于大肠腺瘤(10.0%,6.67%)和正常大肠黏膜(0%,0%),且与大肠癌组织分化程度、浸润深度、淋巴结转移、Dukes分期有关。APC蛋白表达与大肠癌组织分化程度有关。大肠癌中β-catenin异位表达与APC阳性表达呈负相关关系;β-catenin与E-cadherin胞膜阳性表达呈正相关关系。结论:APC失表达和(或)β-catenin异位表达与大肠癌的发生密切相关,可能是大肠癌发生的早期事件;β-catenin、E-cadherin膜表达缺失与大肠癌的侵袭、转移有关。更多还原

【Abstract】 Objective:To investigate the role of APC,β-catenin and E-cadherin in the carcinogenesis and progression of colorectal carcinoma.Methods:Expression of APC,β-catenin and E-cadherin proteins was examined immunohistochemically in 30 cases of normal colorectal mucosa,30 cases of colorectal adenoma,10 cases of colorectal adenoma carcinogenesis and 50 cases of colorectal carcinoma.Results:The positive expression rates of APC were 44.0% and 40.0% respectively in colorectal carcinoma and colorectal adenoma carcinogenesis,and both of the rates were significantly lower than that of colorectal adenoma(86.7%) and normal colorectal mucosa(100.0%).The cytoplasmic and nuclear β-catenin expression rates were 62.0%,50.0% and 30.0% respectively in colorectal carcinoma,colorectal adenoma carcinogenesis and colorectal adenoma,and all of the rates were significantly higher than that of normal colorectal mucosa(0%).The cytoplasmic and nuclear β-catenin expression rate in colorectal carcinoma was significantly higher than that of colorectal adenoma.The reduced membranous β-catenin and E-cadherin expression rates were 46.0% and 34.0%,and both of the rates were significantly higher than that of colorectal adenoma(10.0%,6.67%) and normal colorectal mucosa(0%,0%).The reduced membranous β-catenin and E-cadherin expression were closely related with the tissue defferentiation degree,the depth of invasion,lymph node metastasis and Dukes stage in colorectal carcinoma.The cytoplasmic and nuclear β-catenin expression was in negative correlation with the expression of APC.The membranous β-catenin expression was thus in positive correlation with the expression of E-cadherin.Conclusions:The reduced cytoplasmic APC expression,the cytoplasmic and nuclear β-catenin expression may play a pivotal role in carcinogenesis and progression of colorectal carcinoma,and may be an early event.The reduced membranous β-catenin and E-cadherin expression may be related to the invasion and metastasis of colorectal carcinoma更多还原