【摘要】 目的探讨WT-1蛋白在卵巢颗粒细胞瘤(granulosa cell tumor,GCT)发生发展中的作用,研究它和抗苗勒管激素(anti-mullerian hormone,AMH)、雌激素受体(ER)和孕激素受体(PR)在卵巢GCT中的表达及意义。方法用免疫组化方法研究WT-1蛋白、AMH、ER和PR分别在37例卵巢GCT和10例正常卵巢组织中的表达情况。结果WT-1蛋白、AMH、ER在卵巢GCT中的表达都明显高于它们在正常组织中的表达(P<0.05),且随组织分化程度与临床分期提高而降低,而PR的表达在正常组、不同分化组与不同临床分期组之间差异无统计学意义。WT-1蛋白和AMH的表达呈正相关关系,ER和PR的表达亦呈正相关关系。结论WT-1蛋白与卵巢GCT的发生发展无直接关系。它和AMH、ER可以作为卵巢GCT的辅助诊断指标,测定它们的表达可为诊断及判定预后提供理论依据。更多还原

【Abstract】 Objective To investigate the expression of WT-1 protein,anti-mullerian hormone,ER and PR in ovarian granulosa cell tumor,and to explore the role of WT-1 protein in the development of ovarian granulosa cell tumor. Methods Expression of WT-1 protein,AMH,ER and PR was measured with immunohistochemistry in 37 cases of ovarian granulosa cell tumor and 10 cases of normal ovary. Results Expression of WT-1 protein,AMH and ER in ovarian granulosa cell tumor was significantly higher than that in normal ovary(P<0.05),and the expression of them decreased in patients from stage Ⅰ to stage Ⅲ and from well differentiation to poor differentiation.The expression of PR between tissues of normal ovary and ovarian granulosa cell tumor,between different differentiated tissues,and between different stage tissues was not significantly different,respectively.The expression of WT-1 protein was positively correlated with AMH,and ER was positively correlated with PR. Conclusion The WT-1 protein may be not directly involved in the development of ovarian granulosa cell tumor.WT-1 protein,AMH,ER can be served as subsidiary tumor markers,and their expression can provide a theoretical basis for diagnosis and prediction of prognosis of ovarian GCT.更多还原