【摘要】 目的研究人参皂苷Rh2(G-Rh2)对人胃癌细胞SGC-7901的影响。方法采用MTT法检测G-Rh2对SGC-7901细胞存活率的影响;流式细胞分析法检测凋亡小体的含量;免疫印迹技术及体外Caspase-3/-7活力测定方法检测Caspase的激活状态。结果G-Rh2对SGC-7901细胞生长有明显的抑制作用,且呈量-效关系,IC50为9.3μg/ml。7.5μg/mlG-Rh2作用SGC-7901细胞24h,凋亡细胞数量为6.97%。7.5μg/mlG-Rh2作用SGC-7901细胞20h,出现多聚(ADP-核糖)聚合酶[poly(ADP-ribose)polymerase,PARP]断裂,Caspase-3/-7活力开始出现,并随作用时间的延长而增强。结论G-Rh2诱导Caspase参与SGC-7901细胞凋亡。更多还原

【Abstract】 Objective To study the effect of ginsenoside Rh2(G-Rh2) on human gastric carcinoma SGC-7901 cells.Methods Add G-Rh2 to SGC-7901 cells to various final concentrations.Determine the survival rate of SGC-7901 cells by MTT method,the content of apoptotic body by flow cytometry,and evaluate the activation of caspase by Western blot and determination of caspase-3/-7 activity in vitro.Results G-Rh2 showed significantly dose-dependent inhibitory effect on the growth of SGC-7901 cells.The IC50 of G-Rh2 was 9.3 μg/ml.After treatment with 7.5 μg/ml of G-Rh2 for 24 h,the apoptosis rate of SGC-7901 cells was 6.97%.Both the cleavage of poly(ADP-ribose)polymerase(PARP)and caspase-3/-7 activity appeared after treatment with 7.5 μg/ml of G-Rh2 for 20 h and increased as time goes on.Conclusion G-Rh2 induced the apoptosis of SGC-7901 cells in which caspase was involved.更多还原