【摘要】 目的:研究多效生长因子对基质金属蛋白酶(MMP)3、10的负调控作用。方法:用RT-PCR、Northern印迹比较对照与多效生长因子沉默细胞中MMP3、MMP10的表达;在多效生长因子沉默细胞培养液中加入50 ng/mL多效生长因子,检测MMP3、MMP10的表达。结果:多效生长因子缺失细胞中MMP3、MMP10高表达;而在其中添加多效生长因子之后,MMP3、MMP10的表达基本被完全抑制。结论:多效生长因子对MMP3、MMP10有负调控作用。更多还原

【Abstract】 Objective: To investigate the transcription regulation of matrix metalloproteinase (MMP) 3,10 by pleiotrophin (PTN).Methods: The mRNA expression levels of MMP3,MMP10 were compared between control and PTN knocking down MEF cells by RT-PCR and Northern Blot.Then,50 ng/mL PTN was added in culture medium of the PTN knocking down MEF cells to investigate the effect of pleiotrophin on the transcription of MMP3,MMP10.Results: MMP3,MMP10 expressed higher in the cell that PTN was absence,but they expressed lower after the PTN effected.Conclusion:PTN negative regulated the expression of MMP3 and MMP10.更多还原