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苏云金芽孢杆菌蛋白酶发酵动力学模型的构建

Kinetics Model for Protease of Bacillus thuringiensis by Liquid Fermentation

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【作者】 郑毅张志国关雄

【Author】 ZHENG Yi~(1,2) ZHANG Zhi-guo~1 GUAN Xiong~2 (1 College of Life Sciences,Fujian Normal University,Ministry of Education, Fuzhou Fujian 350007 China) (2 Key Laboratory of Biopesticide and Chemical Biology,Fujian Agriculture and Forestry University,Ministry of Education,Fuzhou Fujian 350002 China)

【机构】 福建师范大学生命科学学院福建农林大学生物农药与化学生物学教育部重点实验室

【摘要】 对苏云金芽孢杆菌FS140蛋白酶分枇发酵的代谢特性进行了研究.首先描述了FS140分枇发酵过程中细胞生长、产物积累、糖消耗的变化规律.基于Logistic方程和Luedeking-Piret方程,建立了苏云金芽孢杆菌蛋白酶发酵过程细胞生长、产物合成及基质消耗随时间变化的数学模型.动力学模型计算值结果与实验值拟合良好,较好反映了苏云金芽孢杆菌分批发酵过程的动力学特征.

【Abstract】 study of the metabolic characters of thermophilic protease batch fermentation with Bacillus thuringiens FS140 was conducted.Cell growth,protease production and sugar consumption throughout the protease fermentation were investigated.The kinetic models for Cell growth,protease formation,sugar consumption were proposed according to the Logistic equation and Luedeking-Piret equation.The calculated results of models were compared satisfactorily with the experimental data.The model equations could really reflect the protease fermentation process and kinetic mechanism.Protease formation was partially associated with the growth of Bacillus thurinqiensis FS140.

【基金】 国家高技术研究发展计划(2006AA10A212);福建省科技创新平台建设计划(2007N2010);福建省科技厅资肋项目(2006F5032)
  • 【文献出处】 生物数学学报 ,Journal of Biomathematics , 编辑部邮箱 ,2008年04期
  • 【分类号】TQ925.2
  • 【被引频次】28
  • 【下载频次】450
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