【摘要】 本研究用225对引物对农艺性状优良但对白粉菌敏感的栽培小麦京411、抗白粉病栽培小麦Brock以及京411与Brock配制的近等基因系进行AFLP分子标记筛选,结果发现只有2对引物组合Pst+GAC/Mse+TCT(P1)和Pst+AGC/Mse+ACC(P2)在上述抗感材料中表现出多态性,分别扩增到2个特异片段,将特异片段克隆并测序发现,P1扩增的特异片段长268bp,P2扩增的特异片段长227bp,命名为AFLP标记P1268和P2227。用106个京411×Brock的F2单株进行连锁性分析表明,P1268和P2227与抗白粉病基因的遗传距离分别为3.6和1.9cM,与Brock中的抗白粉病基因呈紧密连锁。该两个AFLP标记对小麦抗白粉病基因的积累和分子标记辅助选择育种有重要意义。更多还原

【Abstract】 Wheat cultivar Jing 411 which is susceptible to powdery mildew,wheat cultivar Brock and near isogenic lines (NILs) of Jing411,which are resistant to powdery mildew were analysized for polymorphisms using 225 pairs of AFLP primers. Only two pairs of primers Pst+GAC/Mse+TCT(P1) and Pst+AGC/Mse+ACC(P2) stably produced polymorphic bands between the resistant and susceptible plants. Two specific fragments were obtained. By cloning and sequencing these two specific fragments,it was showed that the specific fragment amplified by primer P1 had 268bp,and the fragment amplified by P2 had 227bp. They were named AFLP marker P1268 and P2227 respectively. Linkage analysis of these two markers revealed that the polymorphism existed in a 106 F2 segregating population. These two markers closely linked to a powdery mildew resistance gene in wheat cultivar Brock,linkage distance were 3.6 and 1.9cM respectively. These two markers will be useful for marker-assisted selection and gene pyramiding in wheat resistance breeding.更多还原