By means of enzyme analysis,the effects of temperature and pH were examined on the activities of protease, amylase and cellulase in Estellarca olivacea.The results indicated that protease,amylase and cellulase were significant affected by temperature and pH.The optimum temperatures of protease,amylase and cellulase were 60℃,40℃and 30℃~40℃,and the optimum pH value were 3.6,5.2 and 6.0,respectively.The activity of amylase is higher than protease and cellulase.The activity of cellulose is lowest in all three d...