【摘要】 目的:用热休克蛋白Hsp(Heat Shock Proteins)基因重组大肠杆菌,改善细胞生长状况、提高大肠杆菌的逆境耐受性和乙醇产量。方法:将来自Pyrococcus furiosus的基因Hsp与Lac启动子串联,构建成由Lac启动子调控Hsp表达的操纵子,经该操纵子转化的大肠杆菌分别在高渗透压、酸性条件、高温和高糖的条件下发酵,利用气相色谱检测发酵液中的乙醇含量。结果:含有Hsp基因的工程菌与不含Hsp基因的对照菌相比,在0.4mol/L NaCl的高渗透压下乙醇产量提高1.5倍、在pH4.5的酸性条件下提高1.2倍、在高温高糖的条件下提高5.95倍。结论:热休克蛋白Hsp基因的表达可以提高大肠杆菌在逆境中的代谢能力。更多还原

【Abstract】 Objective: E.coil which have Hsps genes can have more ethanol production and better growth in high temperature and high sugars. Method:In this paper Hsp genes was amplified by PCR from Pyrococcus furiosus,and placed under the control of lac promoter.The recombinant plasmids was transformed into E.coil. Ethanol production and the cell surviving were analyzed in high temperature, high sugars, high pressure conditions by GC.Result: the cell which have Hsp genes ethanol production were 1.5,1.2 and 5.95 times than the control’s in the different adversity. Conclusion: heat shock proteins can lead to acquired therm tolerance, allowing the organisms to survive at evenhigher temperatures.更多还原