【摘要】 目的:了解中国新疆吉木萨尔野马繁殖中心的普氏野马(Equus przewalskii)遗传多样性及其遗传背景。方法:采用PCR产物直接测序法,对15匹普氏野马线粒体DNAD-loop高变区进行测序分析。结果:测定15个个体的线粒体DNAD-loop高变区15464~15866片段序列402bp。检测到12种单倍型,包括37个多态位点,占全部序列的9.2%,其中转换位点24个、颠换位点20个、转换位点和颠换并存位点8个、缺失位点3个。A%+T%含量(56.1%)高于G%+C%含量(43.9%),平均A含量为28.4%,T含量为27.7%,C含量为29%,G含为14.9%。单倍型间平均遗传距离为0.030,单倍型多态性(h)为1±0.00116,核苷酸多态性(π)为2.90%。15匹普氏野马线粒体DNAD-loop高变区之间平均核苷酸变异率为2.48%。结论:研究表明我国新疆吉木萨尔野马繁殖中心的普氏野马线粒体DNAD-loop区序列存在丰富的多态性。更多还原

【Abstract】 Objective:To investigate the genetic diversity and genetic data of Przewalski’s horses from Wild Horse Breeding Center in Jimsar county, Xinjiang Province, China. Method: Sequence polymorphisms in 15 individuals of Przewalski’s horses mtDNA D-loop region were determined by PCR production direct sequencing. Result: The mitochondrial D-loop hypervariable segment sequences 15464～15866 (402bp) in 15 individuals of Przewalski’s Horse were sequenced.12 haplotypes were identfied in the samples, with 37 sites showing polymorphism, it accounts for 9.2% in total sequences, which were 24 transitions, 20 transversion, 8 coexistent sites of transition and transversion and 3 deletions. The contents of A%+ T% 56.1 % was higher than C%+ G% 43.9%. The contents of A, T, G and C were 28.4%,27.7%, 14.9% and 29%, respectively. The haplotypes diversity is 1±0.00116. The nucleotide diversity is 2.90%. The average genetic distance of haplotypes is 0.030. The average nucleotide mutational rate of mtDNA D-loop varied region in 15 individuals of Przewalski’s horses was 2.48%. Conclusion: The results showed that the Przewalski’s horses maintain high variation in mitochondrial D-loop sequences.更多还原