【摘要】 目的:比较以确定适用于野生稻总RNA提取的方法。方法:用改良的异硫氰酸胍法、TRIZOL法、SDS法和CTAB法分别提取云南3种野生稻和栽培稻滇超6号在灌浆期颖果的总RNA。结果:SDS法能够提取的普通野生稻和疣粒野生稻的总RNA的纯度高,其A260/A280介于1.9938~1.9267;而TRIZOL法只适用于栽培稻滇超6号,CTAB法对于疣粒野生稻和药用野生稻提取效果一般,电泳条带不很清晰,其A260/A280介于1.8243~1.6928;而新建立的改良的异硫氰酸胍法都能提取完整性好、高得率(浓度介于0.9358~1.2008μg/μl)和高纯度的RNA(A260/A280大于1.8),电泳28S rRNA和18S rRNA条带清晰,且该方法操作简单、耗时少、效率高。结论:改良的异硫氰酸胍法适合于野生稻颖果总RNA的提取,提取的总RNA完整性好、得率高。更多还原

【Abstract】 Objective and Methods:In order to find the best method to isolate the RNA from the caryopses of three species of wild rice and cultivated rice(dianchao-six),the improved Isothiocyanate method,the Trizol method,the SDS method and the CTAB method were utilited and compared in this paper.Results: The purity of the RNA isolated by the SDS method from Oryza.rufipogon and.O.granulata was high and the ratios of A260/A280 were between 1.9938 and 1.9267;while TRIZOL method was only applied to dian-chao-six,the extraction effect of the RNA which isolated from O.granulata and O.officinalis by CTAB method is moderate,and the bands was obscure and the ratios of A260/A280 were between 1.8243 and 1.6928; The RNA isolated by the improved Isothiocyanate method produced two clear bands of 28S rRNA and 18S rRNA,the integrity and purity was good(the ratios of A260/A280 were above 1.8),the output rate,too(concentration ranged from 0.9358 to 1.2008μg/μl).and this method was simple and less time-consuming, high efficiency.Conclusion:The improved Isothiocyanate method suited to the total RNA extraction from the caryopsis of wild rice species,and its good integrity and high output rate.更多还原