【摘要】 研究了采用热水法直接提取酿酒酵母胞内产物S-腺苷-L-甲硫氨酸(SAM)北京,并且通过实验对影响SAM抽提的5个条件:抽提温度、抽提时间、热水用量、搅拌转速、硫酸用量等进行了优化。得出的最佳实验条件为:温度70℃、每30 g湿菌体加入热水100 mL、硫酸浓度0.25 mol/L、搅拌转速160 r/min,此时SAM的抽提率可达91.5%。与其他方法相比,该方法耗时少、仪器简单、抽提液中S-腺苷-L-甲硫氨酸质量浓度高、经济且无污染。更多还原

【Abstract】 A method for extracting S-adenosyl-L-methionine from Saccharomyces cerevisiae with hot water was investigated.Experimental results showed the optimum condition was at temperature of 70℃,the ratio of hot water to wet yeast at 100 mL∶30 g,the concentration of sulfuric acid at 0.25 mol/L,and rotating speed at 160 r/min.Extraction rate reached upto 91.5%.The hot water extraction process featured a convenient,fast,effective,economical and non-contaminated method for the extraction of S-adenosyl-L-methionine.更多还原