【摘要】 目的:研究不同浓度的胰岛素及D-葡萄糖对弓形虫在3T3-F442A细胞内分裂增殖的影响,并确定不同条件下弓形虫的生长活力,探索弓形虫生长最佳条件。方法:采用细胞培养及细胞计数单因素方差分析方法研究不同时间内胰岛素及D-葡萄糖对弓形虫在3T3-F442A细胞内分裂增殖的影响,并采用MTIT方法确定不同条件下弓形虫的生长活力。结果:浓度为10^-2μg/ml¹0^-1μg/ml胰岛素和浓度为4.5 g/L葡萄糖相结合共同作用时,弓形虫3T3-F442A增殖率达到最大,其生长活力为最高。当DMEM中不合D-葡萄糖时,胰岛素则对弓形虫的生长所起的作用较差。结论:胰岛素对弓形虫在细胞中生长具有剂量促进效应,表现在低浓度的胰岛素对弓形虫的生长发育具有促进作用,而高浓度的胰岛素则对弓形虫的生长起抑制作用。D-葡萄糖作为一种能源,也是细胞内弓形虫生长不可缺乏的。更多还原

【Abstract】 Objective:Study the stimulative effects of different concentrations insulin and D-glucose on Toxoplasma gondii replication in 3T3-F442A cells and determination of its activity so as to establish the optimal growth condition of T.gondii.Methods:The tachyzoite concentration was determined by enumeration in a haemocytometer（LEVY counting chamber）.The survival of tachyzoites was examined using an MTT assay.Results were expressed as means ± S.E.M.Significance of the data was evaluated by Student’s unpaired t-test.Results:Insulin concentrations between 10^-2 and 10^-1 mg/ml combination of 4.5 g/1 D-glucose in DMEM medium gave maximum stimulus to T.gondii replication.However,insulin,in the absence of D-glucose,had the least effect on T.gondii growth.D-glucose concentrations significandy affected the tachyzoite replication and appear to be indispensable for maintaining the host 3T3-F442A cells.Conclusion:Insulin and D-glucose have a dose-responsive mitogenic effect on intracellular T.gondii replication and development in 3T3-F442A cells and lower concentrations of insulin have a significant positive effect on T.gondii growth though higher concentrations of insulin have a negative effect on T.gondii replication.However,insulin,in the absence of D-glucose,had the least effect on T.gondii growth.更多还原