【摘要】 采用柱上聚合的方法制备血红蛋白溶液,解决传统血红蛋白聚合过程中由于戊二醛的活性过高导致平均分子量大、产物分子量分布宽的问题。该方法利用阳离子交换剂对修饰度(聚合度)小的血红蛋白吸附能力大的原理,使其在柱上富积,同时加入戊二醛进行聚合反应。结果表明该方法能比较有效地缩小聚合血红蛋白的分子量分布。更多还原

【Abstract】 Poly-hemoglobin is the active component of hemoglobin-based blood substitutes. The excess reactivity of glutaraldehyde with hemoglobin in solution leads to poly-hemoglobin of a wide molecular weight distribution and a high average molecular weight. A new polymerization method has been tested to decrease the molecular weight distribution and the average molecular weight. The poly-hemoglobin with lower degree of modification (polymerization) was found enriched on the cation exchange columns and further polymerized with glutaraldehyde. The poly-hemoglobin of narrower molecular weight distribution has been prepared in this way.更多还原