【摘要】 采用可生物降解的聚合物作为壳材料,血红蛋白(Hemoglobin,Hb),形成纳米尺度的血红蛋白微囊拟用作血液代用品。本实验采用可生物降解聚合物聚己内酯(PCL)作为壳材料,制成粒径在120~200nm的载血红蛋白微囊,当内水相Hb浓度为50g/L时,包封率达99·4%。对天然Hb和Hb微囊的红外分析表明制备过程没有影响Hb的结构。P50为27mmHg表明此载Hb微囊的携氧能力。体内外血液安全性评价包括:浸提液与血清混合培养后,测定了其中C3含量无明显变化,说明无明显补体激活发生。溶血、凝血试验合格。微囊输注小鼠体内后,血小板计数无不良反应。载Hb纳米微囊的制备工艺对Hb的包埋有效,并且从结构和功能上模拟了天然红细胞,体内外血液安全性评价合格,是一种极具开发前景的血液代用品。更多还原

【Abstract】 Hemoglobin-loaded nano-sized particles with oxygen carrying capacity were prepared. All experiments were performed using biodegradable polymer poly(polyε-caprolactone) (PCL) as matrix polymer. Optimized preparation parameters led to nanoparticles with well-defined characteristics such as size <200nm, P50 27mmHg and high encapsulation efficiency up to 99.4%. The results of in vitro and vivo studies suggested that Hb-loaded particles did not activate complements. After the nanoparticles suspension was injected into the mice via tail vein,the particles did not cause significant changes in total platelet counts. Apparently, the hemoglobin-loaded nanoparticles can serve as a potential candidate in substitution for red cells.更多还原