【摘要】 目的:观察麦冬不同提取物对过氧化氢诱导的人脐静脉内皮细胞（HUVEC）间黏附分子-1（ICAM-1）和VEGF、Bc（?）-2表达的影响。方法:体外培养HUVEC,用过氧化氢（H₂O₂）制造HUVEC损伤模型。以四甲基偶氮唑蓝（MTT）比色法检测细胞存活数量,用流式细胞仪检测HUVEC表面ICAM-1的表达量;免疫细胞化学方法检测HUVEC的VEGF、Bc（?）-2的分布情况。结果:模型组较正常对照组细胞增殖活性明显降低（P<0.01）。与模型组相比,经麦冬水提物、正丁醇提取物处理组细胞增殖活性明显增加（P<0.05,P<0.01）。流式细胞仪检测显示正丁醇提取物可降低过氧化氢增加的ICAM-1基因的表达。Bc（?）-2的表达,模型组明显低于正常对照组,而正丁醇组表达明显高于模型组（P<0.01）。VEGF的表达,模型组明显高于正常对照组,麦冬水提物、正丁醇提取物处理组高于模型组（P<0.05,P<0.01）。结论:麦冬提取物具有抗凋亡、促增殖、降低细胞间黏附分子-1表达的作用,尤以正丁醇提取物效果更为显著。更多还原

【Abstract】 Objective:To investigate the influence from different ophiopogon japonicus extractives on expression of intercellular adhesion molecule-1（ICAM-1） and VEGF,Bcl-2 of human umbilical venous endothelial cell（HUVEC） damaged by hydrogen peroxide. Methods:HUVECs were cultured in vitro;HUVEC damage model was manufacturd by hydrogen peroxide （H₂O₂）.Blue methylthiazolyl tetrazolium （MTT） assay was used for measuring the number of survival cell,and flow cytometry was used to analyze the expression of ICAM-lon the surface of HUVEC,and immunocytochemical method was used to detect the distribution of VEGF,Bcl-2 in HUVEC. Results:Compared with the normal control group,the cell proliferation activity of model group was significantly decreased（P<0.01）. Compared with the model group,after treated by water extract and butanol extract from ophiopogon japonicus,the cell proliferation activity was significantly increased （P<0.05,P<0.01）.The measurement of flow cytometry showed that then-butanol extract could reduce the gene expression of ICAM-1 increased by hydrogen peroxide treatment.For Bcl-2expression,the model group was much less than the nomal control group,and ophiopogon japonicus extract group was higher than the model group （P<0.01）.For VEGF expression,the model group was much higher than the normal control group,and ophiopogon japonicus extract group was higher than the model group（P<0.01）.Conclusion:Ophiopogon japonicus extract could be used for anti-apoptotic,promoting proliferation,reducing expression of ICAM-1,especially,the extractive from n-butanol.更多还原