【摘要】 目的:研究WFS1在胰腺发育不同阶段的表达和细胞定位。方法:运用Western Blot技术检测WFS1在大鼠胰腺发育不同阶段的蛋白表达水平;运用免疫荧光检测不同时期WFSl在胰腺的定位。结果:WestemBlot结果显示WFS1的蛋白表达量胚胎后期高于新生期,成年期表达量上升;免疫荧光结果显示在不同发育时期WFS1与胰岛β细胞共表达。结论:WFS1在胚胎发育中后期的高表达可能与胰岛形成及功能完善有关,并且可能参与了胰岛重塑。更多还原

【Abstract】 To investigate the expression and cell location of WFS1 during pancreas development.Methods:The expression of in different development stages of rat pancreas was examined by Western Blot;WFS1 location in pancreas was examined by double fluores- cence immunohistochemistry.Results:The expression of WFS 1 was found much higher in the anaphase of embryo than in the newborn and was rising at the adult stage.WFS1 and pancreatic islets cellβcoexisted and were expressed in the different development stages of rat pancreas.Conclusion:The expression of WFSI was higher in the anaphase of embryo,which may be related to formation and func- tional improvement of pancreatic islets as well as remodeling of pancreatic islets更多还原