【摘要】 对具有茶氨酸生物合成能力的γ-谷氨酰转肽酶(γ-GGT)基因工程菌的发酵培养基进行了优化。首先采用Plackett-Burman实验设计对影响γ-GGT活性的培养基成分进行筛选,然后将筛选得到的葡萄糖、酵母提取物、K2HPO43个关键影响因素进行响应面分析,通过对二次多项回归方程求解得到关键影响因素的最佳浓度为葡萄糖10.39g/L、酵母提取物6.88g/L、K2HPO47.10g/L,γ-GGT活性的最大预测值为4.42U/mL,实验验证值为4.40U/mL。最后通过基因工程菌催化合成茶氨酸反应,得到茶氨酸的产量为32.22g/L。更多还原

【Abstract】 This article studied the optimization of cultivation medium for the genetically engineered Escherichia coli strain of γ-Glutamyltranspeptidase for the biosynthesis of theanine. Plackett-Burman design was undertaken to evaluate the effects of six cultivation medium factors, glucose, yeast extract and K2HPO4 were found to be the critical for the factors activity of γ-GGT. Then response surface methodology was used to optimize the above critical factors, the optimum levels of the factors were glucose 10.39 g/L, yeast extract 6.88 g/L, K2HPO4 7.10 g/L, with a predicted γ-GGT activity of 4.42 U/mL, and the experimental γ-GGT activity obtained was 4.40 U/mL. The yield of theanine from L-Gln and ethylamine was 32.22 g/L.更多还原