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条纹斑竹鲨的核型研究

Karyotypic study on Chiloscyllium plagiosum

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【作者】 谢仰杰翁朝红

【Author】 XIE Yang-jie1,2,WENG Zhao-hong2(1.College of Oceanography and Environmental Science,Xiamen University,Xiamen 361005,China;2.The Key Laboratory of Science and Technology for Aquaculture and Food Safety,Fisheries College,Jimei University,Xiamen 361021,China)

【机构】 厦门大学海洋与环境学院集美大学水产学院省高校水产科学技术与食品安全重点实验室

【摘要】 以条纹斑竹鲨活体为材料,采用体内注射秋水仙素和低渗—空气干燥制片法,对条纹斑竹鲨(Chiloscyllium plagiosum)的染色体组型进行了分析。结果表明,条纹斑竹鲨的二倍体染色体数为2n=104,核型公式为2n=40m+26 sm+8 st+30 t,染色体总臂数NF=170。其中第31对染色体带有随体。对所得结果与相近种类的核型进行比较,表明条纹斑竹鲨在已知核型的软骨鱼类中是二倍体数最多的种类之一,据此可以认为条纹斑竹鲨是比较原始的种类。条纹斑竹鲨的双臂染色体(m和sm染色体)较多,占63.5%,在鲨形总目中是较高的,与其它二倍体染色体数较多的鲨类差异甚大。条纹斑竹鲨的NF也大大高于其它已有NF研究报道的软骨鱼类。

【Abstract】 The karyotype of Chiloscyllium plagiosum was studied by means of injecting colchicines,hypotonic treatment and air-dry technique.The results showed that the chromosome number of diploid cell of C.plagiosum was 104,the karyotype was 2n=40m+26sm+8st+30t,NF=170,and the 31th pair of chromosomes had satellites.Compared to other Chondrichthyes so far studied,C.plagiosum was one of the sharks that has the highest chromosome number,and it suggested that C.plagiosum is a primitive species.C.plagiosum had a high percentage(63.5%) of biarmed elements among Selachomorpha,different from other sharks that had a high chromosome number.Furthermore,its NF was higher than any other sharks so far studied.

【关键词】 条纹斑竹鲨核型染色体
【Key words】 Chiloscyllium plagiosumkaryotypechromosome
【基金】 国家自然科学基金(40776083,40876080);厦门市科技项目(3502Z20063022)
  • 【文献出处】 上海水产大学学报 ,Journal of Shanghai Fisheries University , 编辑部邮箱 ,2008年06期
  • 【分类号】S917.4
  • 【被引频次】2
  • 【下载频次】89
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