【摘要】 采用响应面方法对高产海藻糖的酵母菌的培养条件进行了优化。通过Plackett-Burman(PB)设计法,评价了不同温度、pH、接种量、葡萄糖浓度、酵母抽提物浓度、无机氮源(NH4)2SO4浓度和摇床转速等7个因素对海藻糖产量的影响。筛选出葡萄糖浓度、酵母抽提物浓度以及接种量为海藻糖生产过程中的主要影响因素,而其它因素对生产海藻糖的影响不显著。然后用旋转中心组合设计及响应面分析法确定了主要因素的最优条件,分别为葡萄糖浓度21.2 g/L,酵母抽提物浓度5.25 g/L,接种量10%。更多还原

【Abstract】 Response surface methodology was used to optimize the technology of producing trehalose.The effects of T,pH,inoculation amount,concentration of glucose,concentration of yeast extract powder,concentration of(NH4)2SO4 and rotation speed were evaluated by using Plackett-Burman design.Results showed that the concentration of glucose and yeast extract powder as well as inoculation amount were the main affecting factors while the other factors had no significant effect on the production of trehalose.Then,the central composite design and response surface analysis were used to determine the optimal levels of the main factors.The optimized conditions were as below:concentration of glucose was 21.2 g/L,concentration of yeast extract powder was 5.25 g/L and inoculation amount was10 %.更多还原