【摘要】 从线性和精密度两个方面对纤维蛋白平板法和四肽底物法测定纳豆激酶活性的两种方法进行了比较研究,并验证了两种方法测定值之间的直线相关性。结果表明:纤维蛋白平板法和四肽底物法分别在10.77IU/mL～80.73IU/mL和0.03U/mL～0.09U/mL浓度范围内呈线性,且日间和日内变异系数均小于10%。两者结果具有直线相关性,其关系为纤维蛋白平板法测定值(IU)=264.87×四肽底物法测定值(U)-19.633,(R2=0.9942)。结论:纤维蛋白平板法与四肽底物法均能用于纳豆激酶活性测定,前者测定结果直接,但四肽底物法更灵敏,还可应用于高通量筛选。更多还原

【Abstract】 This study compared the linearity and precision of fibrin plate (FP) method with tetra-peptide (Suc-Ala-Ala-Pro-Phe-pNA) substrate (SS) method, and their results’ correspondency was also investigated. Results showed that: The activity linear ranges were 10.77 IU/mL~80.73 IU/mL for FP method and 0.03 U/mL~0.09 U/mL for SS method respectively, while both CVs were less than 10% in within-day and day-to-day tests. The two results showed a good linear correlation and could be converted as: FP values (IU) = 264.87 × SS values (U) - 19.633, (R2 = 0.994 2). Therefore: fibrin plate method and tetra-peptide substrate (SS) method are both applicable for detecting Nattokinase activity. The result from FP method can directly reflect nattokinase’s thrombolytic activity and the tetra-peptide substrate method has the advantage for fast, sensitive and high-throughput screening.更多还原