【摘要】 采用薄膜-超声法制备番茄红素纳米脂质体,并以纳米脂质体包封率为主要评价指标,采用正交设计法优化番茄红素纳米脂质体的配方。结果表明:番茄红素纳米脂质体的最佳配方比为:番茄红素:胆固醇:卵磷脂=2:15:100;最佳水合介质是pH7.0的PBS缓冲溶液;最适洗膜温度34℃。按该工艺组合制备3批番茄红素纳米脂质体,包封率的平均值为49.88%±0.19%,载药量为0.86%±0.1%,平均粒径小于40nm。按优化工艺可制得包封率稳定、粒径较小、分布均匀的微球体番茄红素纳米脂质体。更多还原

【Abstract】 Objective:To prepare lycopene nanoliposomes. Methods:Lycopene nanoliposomes were prepared by conventional rotary evaporated film-ultrasonication. Taking the entrapment efficiency of lycopene nanoliposomes as the main evaluation index,the prescription of the lycopene nanoliposomes was optimized by orthogonal design. Results:The best ratio of lycopene to cholesterol to phospholipids is 2:15:100. The optimum pH value of the water phase phosphate buffer solution is 7.0 and the temperature is 34 ℃. With the optimized operation,the average entrapment efficiency of nanoliposomes is 49.88 %± 0.19%,the drug is 0.86% ± 0.1%,and the particle size is below 40 nm. Conclusion:The optimized operation is reasonable and practicable to pepare lycopene nanoliposomes with low particle size and homogeneous microspheres shape.更多还原