【摘要】 通过对笃斯越橘花青素进行RP-HPLC分析,首先得到花青素提取率随盐酸浓度变化的规律,并得出盐酸-乙醇法提取花青素的最佳盐酸浓度为1.5mol/L。分别采用AB-8大孔树脂、聚酰胺树脂及AB-8大孔树脂与聚酰胺树脂联用对提取液进行了初步纯化,得到了4种纯化花青素样品,经过RP-HPLC法检测分析获得了4种纯化样品的花青素的纯度分别为13.36397%、36.95687%、13.21466%、8.559135%,可知采用聚酰胺树脂对笃斯越橘花青素的纯化效果明显优于AB-8树脂,纯度可提高23.5%。更多还原

【Abstract】 Depending on the detection of RP-HPLC,the change trend of the extraction rate of anthocyanins from Vaccinium uliginosum L. with the hydrochloric acid concentration was investigated. The best hydrochloric acid concentration is determined as 1.5 mol/L. The Vaccinium uliginosum L.extracts were purified by AB-8 macroporous resin,polyamide resin (eluted with 80% or 40% ethanol solution) and combination of the two resins,respectively,and four anthocyanins samples were obtained and then detected by RP-HPLC. The results showed that the anthocyanins purity of the four samples are 13.36397%,36.95687%,13.21466% and 8.559135%,respectively. According to the above results,the polyamide resin is apparently better than AB-8 macroporous resin in the purification of anthocyanins,and the anthocyanins purity is increased by 23.5%.更多还原