【摘要】 目的:本实验研究了尼生素协同超高压处理对枯草芽孢杆菌存活率的影响。方法:采用响应曲面方法中的Pentagonal模式,对超高压处理枯草芽孢杆菌进行了实验优化设计,并进行了实验分析。结果:(1)压力、尼生素是超高压灭活枯草芽孢杆菌显著影响因子;(2)在本实验条件范围内建立的超高压杀灭枯草芽孢杆菌的回归模型有效,并可用于预测本实验条件范围内以及附近取值的枯草芽孢杆菌超高压杀菌结果;(3)添加尼生素比增加温度能更有效地协同超高压杀灭枯草芽孢杆菌;(4)在添加0.05mg/ml的尼生素基础上继续增加尼生素浓度,杀灭枯草芽孢杆菌效果不断提高,但增加速度不明显。更多还原

【Abstract】 Objective:To investigate the effect ultra high pressure processing(UHPP) combined with nisin on B. subtilis AS 1.140. Method: All experiments were designed according to pentagonal concept of RSM. Result: The pressure and nisin concentration are the most significant factors affecting UHPP sterilization to Bacillus subtilis,and their significance order is pressure>nisin concentration; The regression equation (model) for UHPP sterilization to B. subtilis was established,and also verified to be good for prediction in the range of experimental conditions; Nisin can be more effective to synergize sterilization of UHPP to B. subtilis than temperature; The increase of sterilization rate (the change of log-circles) is higher in nisin concentration of 0.00～0.05mg/ml than in that of 0.50～0.15mg/ml.更多还原