【摘要】 本实验利用DB-Dioxin毛细管色谱柱和GC-ECD成功建立了鱼肉组织中2,3,7,8-四氯代二苯并-P-二噁含量的检测方法。采用Bio-beads S-X3凝胶色谱柱和多色谱纯化对样品进行前处理,以DB-Dioxin毛细管色谱柱进行分离,ECD对鱼肉组织中的2,3,7,8-TCDD进行检测。结果该方法在1.5～100pg范围内呈线性关系(R2=0.9999),标准回收率为103.2%±3.04%,变异系数为2.95%,其最低检测限为1pg。2,3,7,8-TCDD和其衍生物及样品中的杂质分离良好。更多还原

【Abstract】 The study aimed to set up a method of GC-ECD(electron capture detector) and DB-Dioxin capillary chromatographic column for the detection of 2,3,7,8-TCDD in fish meat. Sample was purified by Bio-beads S-X3 GPC,separated in DB-Dioxin capillary column and detected by GC-ECD. The results showed that the detecion of 2,3,7,8-TCDD has better linearity in the range of 1.5 to 100 pg(R2=0.9999). The average standard recovery rate is 103.2%±3.04% with variation coefficient 2.95%. The detection limit is 1pg. It was demonstrated that 2,3,7,8-TCDD is well separated from sample.更多还原