【摘要】 采用高效液相色谱法测定食品中的辣椒素、二氢辣椒素。超声波辅助甲醇-四氢呋喃(1+1)提取样品中的辣椒素、二氢辣椒素,荧光检测器对其进行检测(Ex=229nm、Em=320nm)。结果表明,辣椒素含量在0.13～160mg/L之间,二氢辣椒素含量在0.04～160mg/L之间;峰面积与待测物浓度呈良好的线性关系,相关系数为1;RSD为1.36%～4.79%,回收率在70.50%～100.07%之间。更多还原

【Abstract】 A method was established for capsaicin and dihydrocapsaicin determination by high performance liquid chromatog-raphy (HPLC). Capsaicin and dihydrocapsaicin were extracted by ultrasonic with methanol-tetrahydrofuran (1+1) as solvent, and then detected by HPLC with FLD (Ex=229 nm, and Em=320 nm). A good linear relationship between peak area and concentration of capsaicin (in the range of 0.13～160 mg/L) or dihydrocapsaicin (in the range of 0.04～160 mg/L) exists. The relative standard deviations are between 1.36% and 4.79%, and the recovery rates are in the range of 70.50%～100.07%.更多还原