【摘要】 本实验利用毛细管电泳(CE)化学衍生荧光检测技术建立了食品黑色素的分析新方法。分别研究了诸项因素对化学衍生效率及色谱分离效果的影响。结果表明,在优化条件下,样品分析总时间小于15min,保留时间和峰面积的日间、日内精密度和准确度的相对标准偏差(RSD)均小于4.7%。说明所建立的方法稳定、可靠。与传统的HPLC电化学检测方法相比,本方法的检测灵敏度至少提高了100倍。利用该方法定量分析了四种食品黑色素的含量,样品回收率为92%～97%,结果令人满意。更多还原

【Abstract】 In this work, the suitability of capillary chromatography for the quantitative analysis of melanin in foods was investigated. Various parameters affecting derivatization and separation were systematically studied. Under optimal conditions, the analytes could be separated within 15 min, and the relative standard deviations (RSD) of migration time and corrected peak areas were less than 4.7%. Compared with the conventional high-performance liquid chromatography (HPLC) method with electrochemical detection, the 100-fold improvements in sensitivity were achieved by applying LIF detection. As a preliminary application, this method has been successfully applied to the quantitative determination of melanin in four foods with the spiked recoveries in the range of 92%～97%.更多还原