【摘要】 选用7种14株产桔霉素红曲菌,运用基因组PCR分析方法,从它们的基因组中分别扩增到两条大小分别为669bp和591bp的pksCT基因翻译起始区部分序列和终止密码子区部分序列片段。扩增产物的测序结果通过NCBI进行BLAST同源性分析。结果显示扩增产物序列之间具有高度同源性,且分别与Genbank中公布的紫色红曲菌中pksCT基因翻译起始区部分序列和终止密码子区部分序列一致。pksCT基因是红曲菌中普遍存在的生物合成桔霉素基因。运用基因组PCR分析pksCT基因,是鉴别红曲菌产毒株的有效方法之一。更多还原

【Abstract】 pksCT gene was a related gene responsible for citrinin biosynthesis in Monascus purpureus. In this study, fourteen citrinin-producing Monascus spp. coming from seven Monascus species were used in the experiment. Two DNA fragments from the transcriptional start region and the stop codon region of pksCT gene, which were 669 bp and 591 bp respectively, were gained from fourteen Monascus spp. by genomic PCR. Results of NCBI BLAST showed that PCR products exhibited high identity with each other, and were in accordance with the partial sequences of pksCT gene in M.purpureus which was announced in the Genbank. It was suggested that pksCT gene was a general gene responsible for citrinin biosynthesis in Monascus species. It was also indicated that it was the effective way to distinguish the citrinin-producing Monascus strain by analyzing the pksCT gene with genomic PCR.更多还原