【摘要】 运用传统分离培养方法和RISA图谱分析方法,结合序列测定技术对绍兴黄酒麦曲中真菌的多样性进行了研究。通过纯培养的方式对绍兴黄酒麦曲进行分离,并运用ITS序列分析技术对获得的不同菌株进行了分类鉴定,结果显示在分离获得的16种丝状真菌中,伞枝犁头霉、米根霉、微小毛霉、米曲霉、烟曲霉是麦曲中的主要真菌。RISA图谱分析结果表明这一非培养方法可应用于麦曲中主要真菌的组成研究。此外,运用RISA图谱技术比较了位于不同区域工厂的麦曲真菌多样性,结果显示不同生产区域的麦曲中优势真菌的组成不同,证实周边地域环境是影响麦曲中真菌多样性的重要因素。更多还原

【Abstract】 Fungal diversity of wheat Qu of Shaoxing rice wine was studied by conventional dilution plate method and a culture-independent method RISA (ribosomal intergenic spacer analysis), combining with sequencing. Sixteen kinds of filamentous fungi were separated from wheat Qu and identified by internal transcribed spacer sequencing technology. Among all cultures, Absidia corymbifera, Rhizopus oryzae, Rhizomucor pusillus, Aspergillus oryzae and Aspergillus fumigatus were major fungis in wheat Qu. The results of RISA fingerprint analysis showed this culture-independent method could be used to study fungal diversity of wheat Qu. Different kinds of wheat Qu from three rice wine factories were compared by RISA fingerprint analysis method. The results showed the main fungi in wheat Qu from three districts were different obviously, and proved that regional environment factors influenced the wheat Qu fungal community significantly.更多还原