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肋脉羊肚菌液体培养条件的研究

Study on Liquid Cultivating Conditions of Morchella costata (Vent.) Pers.

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【作者】 杨芳王新风朱骏李刚刘艳文袁生

【Author】 YANG Fang1,2,WANG Xin-feng2,,ZHU Jun2,LI Gang2,LIU Yan-wen2,YUAN Sheng1 (1.Key Laboratory of Microbial Engineering, College of Life Sciences, Nanjing Normal University, Nanjing 210097, China;2.Institute of Resource Microorganism, Department of Biology, Huaiyin Teachers’ College, Huaian 223300, China)

【机构】 南京师范大学生命科学学院微生物工程重点实验室淮阴师范学院生物系资源微生物研究所南京师范大学生命科学学院微生物工程重点实验室 江苏南京210097 淮阴师范学院生物系资源微生物研究所江苏淮安223300江苏南京210097

【摘要】 对肋脉羊肚菌进行液体摇瓶培养,以菌丝干重、胞内多糖产量及其得率为筛选指标,得到最适合肋脉羊肚菌生长的培养基条件为可溶性淀粉浓度2%、豆粕粉浓度1%、MgSO4·7H2O0.1%、KH2PO40.05%、初始pH7.5、培养温度28℃。培养8d后菌丝干重达16.94g/L,胞内多糖量达0.984g/L。

【Abstract】 The optimization of culture of Morchella costata (Vent.) Pers on liquid culture was studied. With dry weight of mycelium, inopolysaccharide(IPS) output and its yield. The results showed that the concentration of starch was 2%, the chorma of lobster sauce was 1%, the concentration of MgSO4·7H2O was 0.1% and the concentration of KH2PO4 was 0.05%, the initial pH of culture was 7.5, and the temperature was 28 ℃. The dry weight of mycelium reached 16.94 g/L, and the weight of inopolysaccharides(IPS) reached 0.984 g/L after culturing for 8 days.

【基金】 江苏省教育厅高新产业项目(JHZD04-006);淮安市科技局项目(SN0635);淮阴师范学院教授基金项目(2006012)
  • 【分类号】S646
  • 【被引频次】8
  • 【下载频次】362
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