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腌制藠头发酵过程中乳酸菌的鉴定及生物学特性比较研究
Identification and biological characteristics of lactic acid bacteria from the natural fermentation of Allium bakei
【摘要】 从传统自然腌制的藠头发酵液中,分离得到七个乳酸菌株,进行了形态学、生理、生化鉴定及生物学特性分析。结果表明:短乳杆菌具有较强的产酸能力和良好的生长特性,在30℃下培养16h,发酵液的pH从7.0降至4.22,活菌数达到2.2×109cfu/mL;短乳杆菌具有较强的耐盐性,且无蛋白分解活性,具有较强的亚硝酸盐降解能力,发酵36h后,能使含量为125mg/L的亚硝酸盐全部转化,说明短乳杆菌具备作为腌制藠头用发酵剂的优良特性。
【Abstract】 Seven species of lactic acid bacteria were isolated from fermentation liquor gotten.The morphologic,physiological,biochemical and biological characters were analyzed and identified by means of tradition natural fermentation.The results showed that Lactobacillus brevis had two features.First,Lactobacillus brevis had strong ability to produce acid.When Lactobacillus brevis was cultured under the condition of 30℃ for 16h,the pH decreased from 7.0 to 4.22 and the number of viable cells increased to 2.2×109cfu/mL.Secondly,Lactobacillus brevis had the property to endure high salt and the ability to reduce the nitrate concentration,but it could not denature protein.Lactobacillus brevis could completely transfer nitrite within 36h when the concentration of nitrate was lower than 125mg/L.Lactobacillus brevis has a good characteristic as a leaven in Allium bakei.
【Key words】 Allium bakei; lactic acid bacteria; identification; fermentation;
- 【文献出处】 食品工业科技 ,Science and Technology of Food Industry , 编辑部邮箱 ,2008年05期
- 【分类号】TS201.3
- 【被引频次】3
- 【下载频次】248