【摘要】 对鼠李糖乳杆菌发酵生产L-乳酸的发酵培养基组成进行了研究;通过正交实验得到最佳氮源组合为酵母粉5g/L,蛋白胨10g/L,牛肉膏15g/L;生长因子实验表明,番茄汁和白菜汁的添加量各为10%时,L-乳酸的产量最高;葡萄糖、吐温80、生长因子和氮源的四因素正交实验表明,最佳培养基组成为葡萄糖160g/L,酵母粉5g/L,蛋白胨10g/L,牛肉膏15g/L,吐温801mL/L,番茄汁50mL/L,白菜汁50mL/L。用该培养基发酵所得L-乳酸的产量高达143.6g/L,得率为89.8%。更多还原

【Abstract】 Culture media composition of L-lactic acid fermentation by Lactobacillus casei subsp.rhamnosus was studied.By orthogonal test,the optimum combination of nitrogen resource were as follows:yeast extract 5g/L,peptone 15g/L and beef extract 15g/L.Tomato juice and Chinese cabbage juice were added into the culture media,the results showed that the concentration of L-lactic acid was the highest level when both of the dosage were 10%.The orthogonal test was also used to get the optimum composition of culture media,according to the experiments,optimized composition of culture media were that glucose 160g/L,yeast extract 5g/L,peptone 10g/L,beef extract 15g/L,Tween 80 1mL/L,tomato juice 5mL/L,Chinese cabbage juice 5mL/L.The maximum concentration of L-lactic acid was 143.6g/L with the yield 89.8%.更多还原