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橄榄果实单宁的抗氧化能力研究

Study on the Antioxidant Activity of Tannins from Canarium album Fruits

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【作者】 张亮亮林鹏林益明

【Author】 Zhang Liangliang~1 Lin Peng~(1,2) Lin Yiming~(1,2) 1 (Department of Biology,School of Life Sciences,Xiamen University,Xiamen 361005,China) 2 (Research Centre for Wetlands and Ecology Engineering,Xiamen University,Xiamen 361005,China)

【机构】 厦门大学生命科学学院生物系

【摘要】 对橄榄果实中的总酚含量与可溶缩合单宁含量进行了测定,并利用二苯基苦基苯肼自由基(DPPH)和铁离子还原/抗氧化能力测定(FRAP)法,研究了橄榄果实中单宁的自由基清除能力及抗氧化活性。结果表明:橄榄果实中总酚含量较高[(572.35±39.72)mg/g(干重)],用正丁醇-HCl法测得缩合单宁的含量为(1.47±0.02)mg/g(干重),用DPPH法和FRAP法研究其自由基清除能力及抗氧化活性表明,橄榄果实单宁具有较高的自由基清除能力(IC50为48.45μg/mL),及较强的抗氧化能力(2.955 mmol AAE/g)。

【Abstract】 Total phenolics and extractable condensed tannins contents of Canarium album Lour.Rauesch. fruits were determined.In addition,the effects of tannins from C.album fruits on free radical—scavenging and antioxidant activity were determined by using 1,1-diphenyl-2-picryhydrazyl (DPPH) radical scavenging activity and ferric reducing/antioxidant power (FRAP) model systems.The results showed as follows,total phenolics and extractable condensed tannins were 572.35±39.72 mg/g and 1.47±0.02 mg/g respective- ly;tannins extracted from C.album fruits showed a very good DPPH radical scavenging activity (IC50 of 48.45μg/mL) and ferric reducing/antioxidant power (2.955 mmol/L AAE/g).The DPPH radical scaven- ging activities were well proved with the ferric reducing/antioxidant capacity of the studied tannins.

【关键词】 橄榄果实单宁多酚DPPHFRAP
【Key words】 Canarium album fruitstanninspolyphenolDPPHFRAP
【基金】 国家自然科学基金(40376026,30671646)
  • 【文献出处】 食品与发酵工业 ,Food and Fermentation Industries , 编辑部邮箱 ,2008年07期
  • 【分类号】R151
  • 【被引频次】19
  • 【下载频次】426
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