【摘要】 目的:探讨SP是否影响培养的大鼠AP细胞[Ca2+]i,在第二信使信息转导途径上进一步阐述SP产生生物学效应的机制。方法:原代培养成年雌性S-D大鼠的AP细胞48h,加入SP孵育不同时间,采用Fura-2/AM检测[Ca2+]i。结果:当SP浓度为100nmol/L,孵育时间由10min增加到30min时[Ca2+]i增加,但孵育时间为40min、50min时,[Ca2+]i呈现减少趋势,即最大反应的孵育时间为30min。当孵育时间为20min、30min、40min、50min时,[Ca2+]i分别为211±16nmol/L、369±51nmol/L、358±24nmol/L、239±36 nmol/L,与10min(117±17nmol/L)相比较,呈显著性差异(P<0.05)。结论:通过Fura-2/AM可精确反映[Ca2+]i,SP兴奋AP细胞SPR后可通过Ca2+来完成其部分的生物学效应,说明了SP对生殖轴(下丘脑-垂体前叶-卵巢/睾丸)有调控作用。更多还原

【Abstract】 Objective:This thesis was to determine whether SP had an effect on the free Ca2+ concentration in primary cultured AP cells of the rat.We farther indicated that SP induced the action mechanism of physiology effect in the way of information transforming of second messengers.Methods:We chose adult female S-D rat AP culture 48h and added SP for different incubating time.After incubating Fura-2/AM was loaded as calcium fluorescent probe.The changes of Ca2+ in AP cells were observed by cation meaturement system.Results:The value of Ca2+ increased in a time-dependent manner with 100nmol/L SP from 10min to 30min while decreased at 40min and 50min.The value of Ca2+ was 211±16nmol/L,369±51nmol/L,358±24nmol/L,239±36nmol/L respectively from 20min to 50min.A significant difference was showed comparing to 10min group(117±17nmol/L)(P<0.05).Conclusions:The system can accurately reflect the concentration of free Ca2+ in AP cells.Biological responses following activation of SPR are mediated by the second messenger Ca2+.The theory of SP controlling the reproducing axis is confirmed in the way of information transforming.更多还原