【摘要】 采用TLC和GC相结合的方法,建立了一套测定生物转化介质中酶解产物没食子酸积累浓度的分析法。结果表明,生物转化液体反应混合物中酶解产物没食子酸积累浓度在4.0-30.0mg/mL范围内,线性关系良好(γ=0.9997),具有较好的准确度和精密度,适用于滇橄榄果汁澄清和果酒"去沉淀"的生物催化和酶法加工过程分析。本法具有简便可行,灵敏可靠的特点。更多还原

【Abstract】 A Simple, available, sensitive and accurate thin-Layer and Gas method has been developed for the determination of the enzymatic lysated product, Gallic Acid (GA) bioconversionnal media. The method requires several initial experimental steps, including sample separation by TLC, elution of chromatogram spot and drying, chemical dorivitization and GC development on one quartz capillary column. All systems provide good separation of the GA, enzymatic lysated in reaction media. Quantitative analyses of GA by GC were accurate and precise with a good linearity ( =0.9997) within the range of 4.0-30.0mg/ml of GA. The GC method was applied to studies of the microbial enzyme methods for clarification of the fruit juice and derecipitation of the wine with Phyllanthus emblica L.更多还原