【摘要】 目的观察甲壳低聚糖(COS)对离体培养的THP-1源性巨噬细胞脂质转运相关基因表达的影响,以初步探讨COS降低血脂的可能分子机制。方法离体培养人THP-1单核细胞,诱导分化成巨噬细胞后,用100μg/mL的COS处理THP-1源性巨噬细胞6,12,24,48 h,运用半定量RT-PCR方法检测其LDL受体、SR-BⅠ、ABCA1和CD36mRNA表达水平的变化。结果COS处理THP-1源性巨噬细胞24和48 h后,LDL受体mRNA的表达与对照组比较分别下调了15.6%(P<0.05)和30.7%(P<0.01);处理6,12,24,48 h后,SR-BⅠmRNA的表达与对照组比较分别上调了14.7%,23.0%,14.6%(P<0.05)和33.9%(P<0.01);处理6和12 h后,ABCA1 mRNA的表达与对照组比较分别上调了12.6%和14.5%(P<0.05);处理6和12 h后,CD36 mRNA的表达与对照组比较分别下调了25.5%和10.2%(P<0.05)。结论通过细胞离体培养实验表明,COS具有的降低血脂作用可能与其能上调巨噬细胞中AB-CA1和SR-BⅠmRNA表达水平以及降低LDL受体和CD36 mRNA水平有关。更多还原

【Abstract】 Purpose To investigate the effect of chitooligosaccharides(COS) on expression of lipid-traffic related genes in THP-1-derived macrophages in vitro,so as to clarify the mechanism that COS could decrease blood lipid level.Methods THP-1-derived macrophages were cultured and treated with COS(100 μg/mL) for 6,12,24 and 48 h respectively.Then LDL receptor,SR-BⅠ,ABCA1,and CD36 mRNA expression were determined by semi-quantity RT-PCR.Results Compared with control,COS significantly increased ABCA1 after 6 h and 12 h and SR-B Ⅰ mRNA expression after 6,12,24 and 48 h,and significantly decreased LDL receptor mRNA level after 24 h or 48 h and CD36 mRNA level after 6 h and 12 h.Conclusion COS could up-regulated ABCA1 and SR-B Ⅰ mRNA level,and down-regulated LDL receptor and CD36 mRNA expression in macrophage cells in vitro,which could be related with the activity of COS decreasing blood lipid level.更多还原