【摘要】 组织工程从诞生至今已有二十年的历史,微载体在组织工程领域的应用研究近年来才日趋升温。利用大孔微载体Cytopore体外构建工程化微构组织,并基于其聚团特性,进行灌注再装配大组织的尝试。结果20 d内微构组织达最高细胞密度16.4×10~7cells/cm~3;32 d后,所形成的微构组织整体均质性好、活性高、基质生成丰富;培养后期微构组织发生明显聚团,经灌注再装配得到细胞和基质分布均匀的厘米级(12mm×6mm)大组织。说明利用大孔微载体构建工程化组织是一种行之有效、富有潜力的体外组织构建方案,有望构建尺寸≥5 mm、细胞和基质分布均匀的大组织。为体外组织构建提供新的思路。更多还原

【Abstract】 Macropomus microcarriers(Cytopore)were used to construct microfabricated tissues.Perfusion approach was used to reassemble macrotissue on the basis of their aggregative property.Microfabricated tissues reached the maximum cell density with 16.4×107 cells/cm~3 after 20 days.They were uniform with high cell viability and massive matrix as a whole after 32 days.The aggre- gation of the microfabricated tissues was visible especially in the later period of the culture,and a centimetre-size(12 mmx 6 mm)macrotissue with uniform cell and matrix distribution was obtained through perfusion reassembly.Constructing engineered tissues using macroporous microcarriers was effective and promising.更多还原