【摘要】 研究瘦素(leptin)对猪骨骼肌成肌细胞分化的影响,并探讨其可能的作用机制。原代培养猪骨骼肌成肌细胞,通过倒置显微镜定性观察细胞分化的形态学变化;肌酸激酶(CK)活性测定法定量分析成肌细胞分化程度;细胞免疫化学方法分析myogenin的表达情况;免疫印迹技术(Western Blot)检测丝裂素活化蛋白激酶(mitogen activated protein kinase,MAPK)的表达变化。结果显示,在猪骨骼肌成肌细胞分化过程中,外源性leptin减少细胞核融合和肌管形成,呈浓度和时间依赖性地显著降低CK活性(P<0.05),并显著抑制myogenin和MAPK的蛋白表达(P<0.05)。以上结果说明,leptin抑制猪骨骼肌成肌细胞分化,并且这种作用可能是通过激活MAPK信号转导通路实现的。研究结果显示,leptin在骨骼肌成肌细胞分化过程中可能具有重要作用。更多还原

【Abstract】 We determined whether body-weight regulator,leptin,could influence differentiation of swine skeletal myoblasts. Myoblast occured in non-leptin and leptin formed in different concentrations(10-1000 ng/mL)for various duration(12-60 h).The morphologic assay demonstrated that leptin significantly decreased the formation of myotubes and myogenic index.In addition,bio- chemical analysis showed that leptin inhibited Creatine Kinase(CK)activity,expressions of myogenin and mitogen activated protein kinase(MAPK).As conclusion,exogenous leptin could inhibit differentiation via MAPK in swine skeletal myoblasts and this sug- gests that leptin be an important mediator in the regulation of muscle-cell differentiation.更多还原