【摘要】 Sef(similar expression to fgf genes)作为FGF信号通路中可诱导的拮抗分子相继在斑马鱼,小鼠,和人类中被鉴定出来,并进行了相应的功能研究.目前对于Sef蛋白本身稳定性的研究还未见报道.对c-Cbl对Sef稳定性的影响进行了研究.免疫荧光实验表明Sef能够和c-Cbl蛋白在细胞中发生共定位,随后的免疫共沉淀实验证明Sef能够和c-Cbl发生相互作用.体内泛素化实验表明c-Cbl能够使Sef发生明显的泛素化作用.这种泛素化最终导致了Sef本身的剂量依赖性的降解.针对c-Cbl的siRNA表达也使Sef稳定细胞系的表达水平得到恢复.结果表明,c-Cbl对Sef的泛素化及降解可能作为一种调控拮抗因子的蛋白质水平从而最终调节信号通路的一种机制.更多还原

【Abstract】 Sef(similar expression to fgf genes) was identified as a feedback antagonist of FGF signaling in zerbrafish,mouse and human.Sef has been reported to function in different ways,however the regulation of Sef stability remains unknown.The possible role of c-Cbl in the regulation of Sef protein degradation was investigated.Results from coimmunoprecipitation and immunostaining assays reveal that hSef colocalizes and interacts with c-Cbl.Data suggest that the interaction between hSef and c-Cbl results in the ubiquitination and subsequent degradation of the hSef protein.It was proposed that c-Cbl may serve as a modulator to regulate Sef protein stability during FGF signal transduction.更多还原