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石岐杂鸡胰岛素样生长因子-Ⅰ基因型与产蛋性状相关性研究

Analysis on the correlation between insulin-like growth factor-Ⅰ genotypes and egg laying performance of Shiqiza yellow hens

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【作者】 俞明月周震祥顾彩菊李震蓝胜芝曹祥荣黄启忠

【Author】 YU Ming-yue1,2,ZHOU Zhen-xiang3,GU Cai-ju3,LAN Sheng-zhi1,2LI Zhen2,3,CAO Xiang-rong1,HUANG Qi-zhong3(1School of Life Sciences,Nanjing Normal University,Nanjing 210097,China;2Shanghai Key Lab of Agricultural Genetics and Breeding,Shanghai 201106,China;3Animal Husbandry and Veterinary Research Institute,SAAS,Shanghai 201106,China)

【机构】 南京师范大学生命科学学院上海市农业遗传育种重点实验室上海市农业科学学院畜牧兽医研究所

【摘要】 采用PCR-RFLP技术对石岐杂黄鸡351个样本的胰岛素样生长因子-Ⅰ(IGF-Ⅰ)基因的5’端调控区进行遗传多态性研究,发现经PstⅠ酶切后出现3种基因型:AA、AB和BB,基因型分布符合哈代-温伯格定律。3种基因型与开产日龄(AFL)、50周龄产蛋量(EP50w)相关性分析结果显示,不同基因型开产日龄差异不显著;50周龄产蛋量,AA型最低,AA型与AB型有显著差异,AA型与BB型差异极显著,BB型与AB型差异不显著。试验结果表明等位基因B可能同IGF-Ⅰ基因表达调控有关,或同产蛋性状基因相连锁。试验为以IGF-Ⅰ基因型为分子标记进行辅助育种研究提供了基础。

【Abstract】 The 351 Shiqiza yellow hens from this institute’s hennery were used as test materials and the genetic polymorphism of the 5’region of their insulin-like growth factor-Ⅰ(IGF-Ⅰ)was studied by means of PCR-RFLP technology.It was found that through PstⅠdigestion,AA,AB and BB three genotypes appeared,and their distribution accorded with the Hardy-Weinberg law.The correlation analysis of the three genotypes with the age at first laying(AFL)and egg production at age 50 weeks(EP50w)showed that there was not a significant difference in the AFL among different IGF-Ⅰgenotypes,but there were significant differences in the EP50w among different IGF-Ⅰgenotypes.The experimental result indicated that the allele B could be related to the IGF-Ⅰgene expression or link with the egg laying performance-related genes.

【基金】 上海市2004年度科技兴农攻关项目(沪农科攻字(2004)第5-5号)资助
  • 【文献出处】 上海农业学报 ,Acta Agriculturae Shanghai , 编辑部邮箱 ,2008年04期
  • 【分类号】S831
  • 【被引频次】6
  • 【下载频次】92
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