【摘要】 选择合适浓度的DMSO溶剂,观察和比较糖苷和苷元对K562细胞增殖的影响.采用MTT法测定DMSO、木犀草素和木犀草苷对K562细胞增殖的影响,对结果进行了统计分析.处理结果显示:DMSO作为溶剂对K562细胞增殖产生影响的最小的浓度为0.1%.梯度实验显示:木犀草素和木犀草苷同它们的抑制作用都有量效关系.结果表明:最好选用0.1%DMSO作为观察药物影响K562细胞增殖实验的助溶剂,浓度偏大或偏小都会表现出它对K562细胞增殖的影响;在0.1%DMSO中,木犀草素和木犀草苷对K562细胞增殖均可表现出抑制作用,且呈浓度依赖性;木犀草苷的作用比木犀草素强;1.15μmol/L的木犀草苷在水溶液中的作用比在0.1%DMSO中的强,且差异极其显著.更多还原

【Abstract】 In a tested compatible concentration of dimethylsulfoxide(DMSO),the effects of luteolin and luteolin-7-O-glucoside on proliferation of K562 cells had been compared.MTT assay was used for the tests,and results were statistically analysed.Results showed that DMSO in 0.1% concentration had less effect on the proliferation of K562 cells than it in other concentrations,which made 0.1% DMSO the most compatible to solve aglycone and glycosides for further researches on their effects on the proliferation of K562 cells.Furthermore the dose-dependency on inhibitory effect was found in both of luteolin and luteolin-7-O-glucoside by testing in the concentrations of 1.15×10-8mol/L,1.15×10-7mol/L and 1.15×10-6mol/L.It could be concluded that 0.1% was the optimum concentration for DMSO being the menstruum.Both Luteolin and luteolin-7-O-glucoside had inhibitory effects on proliferation of K562 cells,but the effect of luteolin-7-O-glucoside might be much stronger.更多还原