【摘要】 目的研究食管癌组织中半胱氨酸蛋白酶8(Caspase-8)基因表达及其与临床参数的关系。方法采用实时荧光定量聚合酶链反应和免疫组化染色法检测96例食管癌患者的食管癌组织和癌旁正常组织Caspase-8表达。结果与正常组织比较,食管癌组织中Caspase-8 mRNA表达者57%下调(P<0.05);食管癌组织Caspase-8蛋白表达显著低于配对的正常组织;Caspase-8 mRNA表达水平与淋巴结转移、家族史、肿瘤体积、肿瘤分期和肿瘤位置有关(P<0.01,<0.05),Caspase-8蛋白表达水平与淋巴转移、肿瘤体积和肿瘤分期有关(P均<0.05)。结论Caspase-8在食管癌发展中起重要作用,对食管癌病情进展的监测、转移潜能及预后估计等具有潜在价值。更多还原

【Abstract】 Objective To investigate Caspase-8 expression levels and the relations between Caspase-8 and clinical parameter in human esophagus carcinomas.Methods We quantified Caspase-8 expression levels in 96 esophageal cancer issues and their matched normal esophagus tissues by using real-time PCR assay and immunohistochemistry staining.Results Caspase-8 mRNA levels of 57% esophageal cancer patients were down-regulated comparing to the matched normal esophagus tissues(P<0.05).The protein expression level of Caspase-8 in esophageal cancer tissues was significantly lower than that in normal esophagus tissues.Caspase-8 mRNA expression levels had significant association with metastasis,family history,tumor size,tumor position and tumor stage(P<0.01,<0.05).Caspase-8 protein expression levels also had significant association with metastasis,tumor size and tumor stage.Conclusions Caspase-8 may play a role in the progression of esophageal cancer,and may have potential value for monitoring pathogenetic progression,metastasis potency and prognosis evaluation.更多还原