【摘要】 目的探讨抗肺纤维化药物对支气管肺泡灌洗液(BALF)中头孢噻肟浓度的影响。方法将120只Wistar雄性大鼠随机分成5组,即正常对照组(A组)、模型组(B组)、干扰素组(C组)、盐酸氨溴索组(D组)、地塞米松组(E组)各24只,除A组外均采用气管内注入博莱霉素的方法制作肺间质纤维化模型。造模后C、D、E组分别每天腹腔内注入γ-干扰素3万U/只、盐酸氨溴索7mg/只、地塞米松0.1mg/只。各组分别于给药后第7、14、28天,每次取6只大鼠,鼠尾静脉注射头孢噻肟600mg/kg后行支气管肺泡灌洗,另各组剩余6只于第28天分别尾静脉注射头孢噻肟1200mg/kg亦行支气管肺泡灌洗,取BALF采用液相色谱—质谱联用法测定头孢噻肟浓度,并取肺组织行病理观察。结果第7天D组、E组BALF头孢噻肟浓度低于B组,C组高于B组,E组与B组有显著性差异(P=0.012)。第14天D组、E组BALF头孢噻肟浓度升高,而C组下降,三组均高于B组,D组与B组有显著性差异(P=0.000)。第28天E组头孢噻肟浓度继续升高,明显高于其他各组,而D组锐降,C组继续下降,且均低于B组,C组、E组与B组有显著性差异。结论地塞米松可提高纤维化期BALF中头孢噻肟的浓度,干扰素、盐酸氨溴索则无。更多还原

【Abstract】 Objective To observe the effect of anti-fibrosis drug on the concentration of cefotaxim in bronchioalveolar lavage fluid.Methods 120 Wistar male rats were randomly divided into five groups,including normal control group(Group A),model group(Group B),interferon-γ-treated group(Group C),ambroxol-treated group(Group D)and Dexamethason-treated group(Group E).On experimental day(day 0),the rats were intratracheally instilled with bleomycin(5 mg/kg)or sterile saline,and then treated with the interferon-γ,ambroxol(35 mg/kg),Dexamethason(0.5 mg/kg)or sterile saline intraperitoneally respectively.On day 7,14,28 after instillation,six rats of each group were sacrificed and the bronchialalveolar fluid was recovered and the lungs were got for histopathological examination after injected cefotaxim(600 mg/kg or 1 200 mg/kg)intravenously.The concentration of cefotaxim in the bronchialalveolar fluid was assayed by liquid chromatography-mass spectrometry method.Results On day 7,the concentrations of cefotaxim in the bronchialalveolar fluid of Group D,Group E were lower than Group B and those of Group C higher than Group B.The difference between Group E and Group B was significant statistically(P<0.05).On day 14,the concentration of cefotaxim in the bronchialalveolar fluid of Group D,Group E increased,while Group C decreased.The three groups were all higher than Group B.The difference between Group D and Group B was significant statistically(P<0.01).On day 28,the concentration of cefotaxim in the bronchialalveolar fluid of Group E increased continuously,which was significantly higher than other groups.Group C and Group D decreased continuously,and the concentration of cefotaxim of both groups was lower than Group B.Conclusion Dexamethason can increase the concentration of cefotaxim in the bronchialalveolar fluid of the fibrosis stage,while interferon-γ and ambroxol can not.更多还原