【摘要】 目的观察抗应激处理对慢性应激抑郁大鼠行为、血清白细胞介素-6(Interleukin-6,IL-6)和皮质醇的影响。方法将32只健康雄性Wistar大鼠随机分为正常对照组、抑郁模型组、普萘洛尔组和酚妥拉明组,每组8只。采用21d慢性轻度不可预见性应激,应用药物对大鼠进行抗应激预处理,实验结束时观察各组大鼠的旷场行为,用放射免疫分析法测定其血清IL-6和皮质醇的含量。结果抑郁大鼠的旷场行为呈现明显的抑制性改变,其血清IL-6和皮质醇含量明显升高。酚妥拉明预处理可使应激大鼠的旷场行为表现及血清皮质醇含量接近正常,但不能阻断其血清IL-6的升高。普萘洛尔预处理对应激大鼠无明显保护作用。结论预先阻断α-肾上腺素能受体可在一定程度上增强大鼠对慢性应激损伤的抵抗能力。应激大鼠血清IL-6的上调可能不受α、β肾上腺素能受体的调控。更多还原

【Abstract】 Objective To explore the effects of anti-stress on behavior and serum concentrations of Interleukin-6(IL-6) and cortisol in chronic-stressed rats.Methods Thirty-two healthy Wistar rats were randomly divided into four groups: the control group,the model group,the propranolol group and the phentolamine group,eight in each group.The chronic unpredictable mild stress(CUMS) model was adopted to stimulate depression in humans.Propranolol or phentolamine pretreatment was used as an anti-stress factor.At the end of the experiment,all the rat′s open-field activities were investigated,and serum IL-6 and cortisol concentrations were determined using radio-immunoassays.Results Open-field activities of the model rats had significant depressive changes,with the serum concentrations of IL-6 and cortisol both significantly elevated.Phentolamine pretreatment helped suppress the behavioral changes and cortisol up-regulation in stressed rats, whereas there were no obvious effect on elevation of the serum IL-6 levels.Propranolol had no anti-stress effect.Conclusion α-Adrenergic receptor(AR) antagonist pretreatment could protect chronic-stressed rats to some extent.The upregulation of stress-induced serum IL-6 may not contribute to the activation of AR in rats.更多还原