【摘要】 目的:探讨胃癌标本中p53基因外显子5~8的突变情况及其与胃癌各临床特征的关系。方法:采用银染PCR-SSCP检测30例配对新鲜胃癌手术标本中p53基因外显子5~8的突变。结果:p53基因突变检出率为46.7%(14/30),p53基因外显子5~8的突变率分别为10%、16.7%、23.3%、16.7%;p53基因突变与病人年龄、性别、原发灶部位及肿瘤分化程度之间差异没有显著性意义(P>0.05);但是,p53基因突变与胃癌临床分期、淋巴结转移之间差异有显著性意义(P<0.05),进展期胃癌p53基因突变高于早期胃癌,有淋巴结转移的胃癌组织中p53基因突变高于无淋巴结转移胃癌组织。结论:p53基因突变可能参与了早期胃癌→进展期胃癌的发生发展过程,并可能是胃癌发生淋巴结转移的机制之一。更多还原

【Abstract】 Objective: To investigate the mutations of p53 exon 5~8 in gastric carcinoma biopsies and their relationship with gastric clinicopathologic features.Methods: Silver staining PCR-SSCP method was used to detect the mutations of p53 exon 5~8.Results: The mutation detecting rate of p53 gene was 46.7%(14/30)in the gastric carcinoma samples and the p53 mutation detecting rates in exon 5~8 were 10%,16.7%,23.3% and 16.7% respectively.There were no significant correlation between p53 mutation detecting rate and the patient’s age,sex,the differentiation degree of tumor cells,and the primary lesion’s position(P>0.05 respectively),while the p53 mutation detecting rate was correlated with the clinical classification(early or advanced) and lymph node metastases(P<0.05 respectively).Conclusion: Our study suggests: 1.p53 mutation may contribute to the development of gastric carcinomas from early stages to advanced stages;2.p53 mutation may be one of the mechanisms involved in the lymph node metastases of gastric carcinoma.更多还原