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酶解植物蛋白对建鲤生长、肝胰脏和肠道发育及消化酶活性的影响
Effects of vegetable protein enzymatic hydrolysates on growth,hepatopancreas and intestine development,digestive enzymes of Jian carp (Cyprinus carpio Var. Jian)
【摘要】 试验考察了酶解植物蛋白对建鲤生长性能、肝胰脏和肠道发育及消化酶活性的影响。体质量为(9.18±0.21)g的建鲤900尾,随机分成6个处理组,每组3个重复,每个重复50尾,分别饲喂酶解植物蛋白替代相应完整植物蛋白0、20%、40%、60%、80%和100%的试验饵料,试验期为60d。结果表明,酶解蛋白替代完整蛋白40%时,生长速度最快,饵料系数最低,摄饵量、蛋白和灰分沉积率最高;替代比例在60%以下时,肠重和肠皱襞高度、肠道脂肪酶、碱性磷酸酶、Na+,K+-ATP酶的活性随着替代比例的上升而显著增加;替代比例超过60%以后,生长速度显著降低、饵料系数升高,肠道酶活、皱襞高度、蛋白沉积率下降,而肝胰脏谷草转氨酶活性、血氨浓度和脂肪沉积率升高(P<0.05)。结果表明,适宜比例的酶解植物蛋白能促进建鲤生长、肝胰脏和肠道发育,提高饲料利用率。
【Abstract】 To obtain the function and eligible application of vegetable protein enzymatic hydrolysates(VPEH),900 carps with an average initial weight of(9.18±0.21)g were divided into 6 groups,fed diets which were formulated with VPEH 0,20%,40%,60%,80%,100% of intact protein respectively for 60 d.The results indicated that when the VPEH in diet protein is 40%,the weight gain,feed intake,protein and ash productive values are higher than those of other groups,the feed conversion ration is lower.Intestinal weight and folds height,lipase,alkaline phosphatase and Na+,K+-ATPase activities enhance with VPEH levels increasing,until to 60%(P<0.05).When VPEH is over 60%,the weight gain,protein and ash productive values,enzymes activities and folds height of intestine decrease,while the glutamine-oxaloacetate transaminase in hepatopancrease,ammonia level in plasma and lipid productive values increase(P<0.05).It suggested that VPEH could promote Jian carp(Cyprinus carpio Jian)growth,hepatopancreas and intestine development,decrease feed conversition rate and when we add VPEH 42.25%,44.57% in the diet respectively,the Jian carp’s growth and feed gain achieve the highest level.The study first described the effects of VPEH in the new species Jian carp’s diet,and investigation of the suitable addition in Jian carp diet.
【Key words】 Jian carp(Cyprinus carpio Var. Jian); protein enzymatic hydrolysates; growth; hepatopancreas; intestine;
- 【文献出处】 水产学报 ,Journal of Fisheries of China , 编辑部邮箱 ,2008年04期
- 【分类号】S963
- 【被引频次】18
- 【下载频次】341