【摘要】 为考察杜仲叶对草鱼生长、血清非特异性免疫指标和肌肉品质的影响,在基础饲料(对照组)中分别添加2%4、%杜仲叶粉或0.1%0、.15%杜仲叶提取物,饲养平均体重(425.8±37.6)g的草鱼。经60d饲养,各组草鱼增重率分别为84.3%、92.2%、96.7%、88.2%、91.7%,饲料系数分别为2.01、1.87、1.82、2.03、1.91;与对照组相比,添加4%杜仲叶粉显著提高了草鱼增重率,降低了饲料系数(P<0.05),添加2%杜仲叶粉、0.15%杜仲叶提取物有提高草鱼增重率趋势(P<0.10)。对血清非特异性免疫指标的测定表明,饲料中添加4%杜仲叶粉,0.1%、0.15%杜仲叶提取物,均可极显著提高SOD活性(P<0.01),各组溶菌酶和碱性磷酸酶活性无显著差异(P>0.05)。对肌肉成分的分析表明,各组间肌肉水分、灰分含量无显著差异(P>0.05),但添加0.15%杜仲叶提取物,可显著提高肌肉蛋白、谷氨酸、丝氨酸、丙氨酸、苯丙氨酸、组氨酸、脯氨酸、呈味氨基酸及总氨基酸含量(P<0.05);添加4%杜仲叶粉、0.1%、0.15%杜仲叶提取物均可提高肌肉羟脯氨酸、胶原蛋白含量。对肌肉物理性能指标的分析表明,添加杜仲叶粉或叶提取物均可降低肌纤维直径(P<0.05),其中,添加4%杜仲叶粉、0.1%、0.15%杜仲叶提取物还提高了肌原纤维耐折力(P<0.05),添加2%、4%杜仲叶粉,0.15%杜仲叶提取物降低了肌肉失水率(P<0.05)。上述研究表明,饲料中添加杜仲叶(叶粉或叶提取物)可改善养殖草鱼生长性能、血清非特异性免疫功能和肌肉品质。更多还原

【Abstract】 To investigate the effect of adding Du-zhong Eucommia ulmoides Oliver leaf in diets on growing performance,non-specific immune function and meat quality of grass carp,fish with body weight of(425.8±37.6) g were fed with basal diet without(control group) or with adding 2%,4% leaf powder,0.1%,0.15% leaf extract.After 60 days feeding,the growth rate and FCR of the five groups were 84.3%,92.2%,96.7%,88.2%,91.7%,and 2.01,1.87,1.82,2.03,1.91 respectively.The growth rate and FCR were significantly improved by adding 4% leaf powder compared with control group(P<0.05),and growth rate tended to be improved by adding 2% leaf powder or 0.15% leaf extract(P<0.10).Serum SOD activities were significantly increased by adding 4% leaf powder,0.1%,0.15% leaf extract(P<0.01),but lysozyme and AKP activities were not affected by all the tested groups(P>0.05).In the respect of muscle chemical composition and quality,there were no differences in muscle moisture and ash contents,but muscle protein,Glu,Ser,Ala,Phe,His,Pro,flavor and total amino acids were obviously increased(P <0.05) by 0.15% leaf extract,and hydroxyproline,collagen content of muscle were increased by adding 4% leaf powder or 0.1%,0.15% leaf extract.When determining muscle physical parameters,muscle fibre diameter were decreased by leaf powders or leaf extracts addition(P <0.05),myofiberbreak-resisting force were increased only by 4% leaf powder,0.1% or 0.15% leaf extract additon,and water loss rate was also decreased by 2%,4% leaf powder,or 0.15% leaf extract addition.Results have showed that growth performance,nonspecific immunity ability and meat quality of grass carp could be improved by adding Du-zhong leaf powder or leaf extract in diets.更多还原