【摘要】 用大黄鱼(♀)和鮸(♂)进行属间杂交,获得了56.25%的受精率,45.24%的孵化率和0.65%的鱼苗成活率。对杂交亲本与子代进行了微卫星和AFLP标记的比较分析,结果表明:4个微卫星位点(LYC0003,LYC0006,LYC0007,LYC0008)都可扩增出清晰的亲本差异条带,26个F1个体中均未出现雄鱼特有条带;4对选择性扩增引物(E-ACC/M-CAG,E-AAC/M-CAT,E-AAC/M-CTA,E-AGC/M-CTC)共检出326个AFLP条带,143条母本特异性条带中有132条出现在子代中,94条父本特异性条带中只有18条出现在子代中,子代中另出现了33条非亲条带。F1个体之间的遗传相似系数为0.891,与母本、父本间的遗传相似系数分别为0.853、0.271;F1个体之间的遗传距离为0.116,与母本、父本间的遗传距离分别为0.159、1.307,表明杂交子代与母本大黄鱼之间具有极高的遗传同质性,属异精雌核发育个体。更多还原

【Abstract】 Intergenus hybrids of Pseudosciaena crocea(♀)and Miichthys mijuy(♂)was produced in this study.The results showed that the fertilization rate was 56.25%,and the hatching rate was 45.24%;however,the survival rate was only 0.65% at fry stage.The genetic analysis on the relationship between the F1 progenies and their parents were carried out using AFLP and microsatellite markers.Clear parental specific bands were amplified from all the four microsatellite loci(LYC0003,LYC0006,LYC0007,LYC0008),but none of paternal specific bands were found in all the 26 F1 progenies. In the AFLP results,a total of 312 bands was produced with 4 selective primer pairs(E-ACC/M-CAG,E-AAC/M-CAT,E-AAC/M-CTA,E-AGC/M-CTC),of which 132 were maternal specific bands,94 were paternal specific bands,and 32 were nonparental bands.The average genetic similarity among the F1 progenies was 0.891,genetic distance was 0.116.The average genetic distance between the F1 progenies and their mother was 0.159,while it was 1.307 between them and their father.The results suggested that the F1 progenies were allogynogens for high genetic identity between the F1 and the female parent.更多还原