【摘要】 目的:超声提取玉竹多糖,观察玉竹多糖对小鼠耐缺氧作用的影响,为玉竹的临床应用和药物开发研究提供一定理论依据。方法:超声提取玉竹多糖。70只小鼠随即分为7组,分别灌胃给予生理盐水、普萘洛尔和不同剂量的玉竹多糖,观察小鼠缺氧存活时间和耗氧量。结果:灌胃给予玉竹多糖50,100,200,400,600 mg/kg和普萘洛尔20 mg/kg,使小鼠0~5 m in的耗氧量分别降低14.02%,27.10%,33.64%,14.02%,17.76%和48.60%,5~10 m in的耗氧量分别降低5.04%,17.27%,16.55%,6.47%,16.55%和14.24%。灌胃给予氯化钠注射液、普萘洛尔20 mg/kg和玉竹多糖50,100,200,400,600 mg/kg,小鼠缺氧30 m in存活率分别为0,90%,0,30%,70%,30%,0。普萘洛尔组、玉竹多糖200 mg/kg组,小鼠的缺氧存活时间分别延长63.05%和52.22%。结论:玉竹多糖能明显增强实验小鼠的耐缺氧作用。更多还原

【Abstract】 To use ultrasonic method to extract the polysaccharides of poygonatum odoratum(POP),to study the effect of POP on hypoxia tolerance in mice,and to provide certain surport for clinic supply,exploitation and research.Methods:To use ultrasonic method to extract the POP.sixty mice were divided randomly into six groups and intragastrically administered respectively with saline,propranolol and different dosages of POP.The survival time and oxygen consumption were measured in mice.Results: When mice were intragastrically administered with POP at the dose of 50,100,200,400,600 mg/kg and propranolol at the dose of 20 mg/kg,the oxygen consumption was reduced respectively by 14.02%,27.10%,33.64%,14.02%,17.76% and 48.60% for 0 to 5 minutes,and 5.04%,17.27%,16.55%,6.47%,16.55% and 14.24% for 5 to 10 minutes.The survival rates of mice with hypoxia for 30 minutes were 0,90%,0,30%,70%,30%,0 when receiving saline,propranolol of 20 mg/kg and POP of 50,100,200,400 mg/kg.The survival time was prolonged by 63.05% and 52.22% in mice with hypoxia of propranolol and POP of 200 mg/kg group.Conclusion:POP shows obvious effect in enhancing hypoxia tolerance in experiment mice.更多还原