【摘要】 猪产仔数的遗传力低,常规育种技术对产仔数性状的改变非常有限,所以寻找控制猪繁殖率的主效基因及其遗传标记对提高猪产仔数具有重要意义。本研究采用PCR-SSCP方法,对莱芜猪、鲁莱黑猪、里岔黑猪、鲁烟白猪、新沂蒙黑猪5个山东地方/培育猪种和大约克夏、长白、杜洛克3个引进猪种共八个猪种323头进行ESR和FSHβ基因的多态性分析,并采用最小二乘法分析其对产仔数的遗传效应。结果表明这两个基因的突变位点存在多态性。对于ESR基因,BB型比AA型母猪初产总产仔数和活产仔数分别高1.38和1.15头,而经产总产仔数和活产仔数分别高0.96和0.91头(P<0.05)。对于FSHβ基因,BB型比AA型母猪的初产总产仔数和活产仔数分别高1.23和1.36头,而经产总产仔数和活产仔数分别高0.74和0.82头(P<0.05)。更多还原

【Abstract】 Because of very low heritability in litter size of pig,the improvement of the litter size is limited by the conventional breeding technology.It is very important to find the major gene and the genetic marker related to the litter size.In this study,323 pigs including Laiwu Black,Lulai Black,Licha Black,Luyan White,New Yimeng Black,Yorkshire,Landrace and Duroc were selected to detect genetic variants of ESRand FSHβ genes by PCR-SSCP.The genetic effects of the genes to litter size were analyzed by the least square analysis.Results showed that polymorphism of this two genes is rich in these pig breeds.As a result for ESRthe average TNB and NBA of BB was 1.38 and 1.15 more than AA in the first parity,while 0.96 and 0.91 more than AA in the later parities respectively(P<0.05).For FSHβ,the average TNB and NBA of BB was 1.23 and 1.36 more than AA in the first parity,while 0.74 and 0.82 more than AA in the later parities respectively(P<0.05)更多还原