【摘要】 为了建立在衣藻中快速简便研究外源基因功能的方法,采用玻璃珠转化法将pBI221质粒(35S,Amp,GUS)高效转化至细胞壁缺陷型衣藻品系CW-15(Arg-),通过对转化条件及GUS表达检测条件摸索,发现35S启动子在CW-15中能有效启动GUS表达,且GUS最佳检测时间约为转化后24h,重组细胞先染色后制片更有利于GUS检测.更多还原

【Abstract】 In order to find a way of studying the heterogenous nuclear segments transformed into Chlamydomonas reinhardtii,pBI221 was transformed into Chlamydomonas reinhardtii CW-15,without Arg.It is showed that 35S promoter could naturally cause GUS expression.The best time point of GUS assay was about 24 hours after transformation.Transformant was stained before rooting cells on vitreous slice.It is better than Jefferson’protocol.更多还原